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strains of hmsc  (Lonza)


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    Lonza strains of hmsc
    Strains Of Hmsc, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strains+of+hmsc/strains+of+hmsc/pm33394669-40-5-8
    Average 90 stars, based on 1 article reviews
    strains of hmsc - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Unveiling the basis of antiretroviral therapy-induced osteopenia: the effects of Dolutegravir, Darunavir and Atazanavir on osteogenesis.
    Article Snippet: We utilized three strains of hMSC purchased from Lonza cultured according to the manufacturer’s instructions (Lonza, Basel, Switzerland) and induced to differentiate by an osteogenic cocktail containing 2 x 10-8 M 1α,25-Dihydroxyvitamin D3, 10 mM βglycerolphosphate and 0.05 mM ascorbic acid (Sigma Aldrich, St. Louis, MO, USA) (osteogenic medium, OM).



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    Microscopy images <t>of</t> <t>unstrained</t> and strained <t>hMSCs.</t> (a) Phase-contrast photomicrographs of hMSCs subjected to cyclic uniaxial stretching in different magnitude and duration of stretching. (b) Higher magnification of phase contrast of unstrained and 8% strained hMSCs at 72 h and tenocytes. (c) Confocal laser scanning micrographs showing actin stress fibers (green) and nuclei (blue) of unstrained cells and 4%, 8%, and 12% strained cells at 72 h. The substrate was stretched in the red arrow direction.
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    https://www.bioz.com/product/strains+of+hmsc/phase+contrast+microscopic+images+unstrained+strained+hmscs/pmc06409073-85-6-10
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    strains of hmsc  (Lonza)
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    Lonza strains of hmsc
    Microscopy images <t>of</t> <t>unstrained</t> and strained <t>hMSCs.</t> (a) Phase-contrast photomicrographs of hMSCs subjected to cyclic uniaxial stretching in different magnitude and duration of stretching. (b) Higher magnification of phase contrast of unstrained and 8% strained hMSCs at 72 h and tenocytes. (c) Confocal laser scanning micrographs showing actin stress fibers (green) and nuclei (blue) of unstrained cells and 4%, 8%, and 12% strained cells at 72 h. The substrate was stretched in the red arrow direction.
    Strains Of Hmsc, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/strains+of+hmsc/strains+of+hmsc/pm33394669-40-5-8
    Average 90 stars, based on 1 article reviews
    strains of hmsc - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Microscopy images of unstrained and strained hMSCs. (a) Phase-contrast photomicrographs of hMSCs subjected to cyclic uniaxial stretching in different magnitude and duration of stretching. (b) Higher magnification of phase contrast of unstrained and 8% strained hMSCs at 72 h and tenocytes. (c) Confocal laser scanning micrographs showing actin stress fibers (green) and nuclei (blue) of unstrained cells and 4%, 8%, and 12% strained cells at 72 h. The substrate was stretched in the red arrow direction.

    Journal: Stem Cells International

    Article Title: Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

    doi: 10.1155/2019/9723025

    Figure Lengend Snippet: Microscopy images of unstrained and strained hMSCs. (a) Phase-contrast photomicrographs of hMSCs subjected to cyclic uniaxial stretching in different magnitude and duration of stretching. (b) Higher magnification of phase contrast of unstrained and 8% strained hMSCs at 72 h and tenocytes. (c) Confocal laser scanning micrographs showing actin stress fibers (green) and nuclei (blue) of unstrained cells and 4%, 8%, and 12% strained cells at 72 h. The substrate was stretched in the red arrow direction.

    Article Snippet: Phase-contrast microscopic images of unstrained and strained hMSCs were obtained (Olympus, Japan) in at least four randomly selected sites from our visual field.

    Techniques: Microscopy

    ECM expression on unstrained and strained cells. (a) Comparison of different collagen staining on various mechanical stimuli hMSCs at 72 h and tenocytes as positive control. (b) Immunofluorescence staining of fibronectin and N-cadherin on unstrained and strained hMSC for 6 h or 72 h. The expression of fibronectin and N-cadherin was enhanced by the cyclic stretch and magnitude strain dependent. The substrate was stretched in the red arrow direction. (c) Thicker fibronectin fibrils were formed by cyclic mechanical stimulation.

    Journal: Stem Cells International

    Article Title: Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

    doi: 10.1155/2019/9723025

    Figure Lengend Snippet: ECM expression on unstrained and strained cells. (a) Comparison of different collagen staining on various mechanical stimuli hMSCs at 72 h and tenocytes as positive control. (b) Immunofluorescence staining of fibronectin and N-cadherin on unstrained and strained hMSC for 6 h or 72 h. The expression of fibronectin and N-cadherin was enhanced by the cyclic stretch and magnitude strain dependent. The substrate was stretched in the red arrow direction. (c) Thicker fibronectin fibrils were formed by cyclic mechanical stimulation.

    Article Snippet: Phase-contrast microscopic images of unstrained and strained hMSCs were obtained (Olympus, Japan) in at least four randomly selected sites from our visual field.

    Techniques: Expressing, Staining, Positive Control, Immunofluorescence

    Mechanical stretching altered hMSC surface antigen expression but did not express osteogenesis and adipogenesis. (a) Expression levels of the CD markers of hMSCs cultured in mechanical stretching with different strains. Significance ( p < 0.05) was represented by ∗ which compared to unstrained. n = 3, error bar ± SD. (b) Percentage of multicolour expression for lymphocyte adhesion molecule CD44 and endoglin CD105. Fluorescent expression intensity and area of CD44 and CD105 in (A) 4%, (B) 8%, and (C) 12% strain magnitudes. (c) Representative images of Alizarin Red- and Oil Red O-stained hMSCs: (A) positive Alizarin Red staining on osteogenic medium cultured hMSCs, (B) negative Alizarin Red result on mechanical stimulated hMSCs, (C) positive Oil Red O staining on adipogenic medium cultured hMSCs, (D) negative Oil Red O result on strained hMSCs.

    Journal: Stem Cells International

    Article Title: Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

    doi: 10.1155/2019/9723025

    Figure Lengend Snippet: Mechanical stretching altered hMSC surface antigen expression but did not express osteogenesis and adipogenesis. (a) Expression levels of the CD markers of hMSCs cultured in mechanical stretching with different strains. Significance ( p < 0.05) was represented by ∗ which compared to unstrained. n = 3, error bar ± SD. (b) Percentage of multicolour expression for lymphocyte adhesion molecule CD44 and endoglin CD105. Fluorescent expression intensity and area of CD44 and CD105 in (A) 4%, (B) 8%, and (C) 12% strain magnitudes. (c) Representative images of Alizarin Red- and Oil Red O-stained hMSCs: (A) positive Alizarin Red staining on osteogenic medium cultured hMSCs, (B) negative Alizarin Red result on mechanical stimulated hMSCs, (C) positive Oil Red O staining on adipogenic medium cultured hMSCs, (D) negative Oil Red O result on strained hMSCs.

    Article Snippet: Phase-contrast microscopic images of unstrained and strained hMSCs were obtained (Olympus, Japan) in at least four randomly selected sites from our visual field.

    Techniques: Expressing, Cell Culture, Staining

    The comparison of cell surface topography between the unstrained hMSCs, strained hMSCs, and tenocytes, visualized by AFM. (a) Representative AFM height and deflection scans of unstrained hMSCs and 4%, 8%, and 12% strained hMSCs, and tenocytes. In height images, brighter colour indicates higher distance of substrate. In deflection images, the detailed structure of presumably the stress fiber could be observed with AFM in different cell groups. The direction of uniaxial strain was in the red arrow direction. (b) Young's modulus on the cytoskeleton of the cells subjected to 4%, 8%, or 12% cyclic stretching for 72 h as indicated. The ratio was counted by normalizing to the expression amount of corresponding unstrained groups (indicated as 1). Statistical significance ( p < 0.05) was represented by ∗ relative to the unstrained group. n = 3, error bar ± SD.

    Journal: Stem Cells International

    Article Title: Uniaxial Cyclic Tensile Stretching at 8% Strain Exclusively Promotes Tenogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

    doi: 10.1155/2019/9723025

    Figure Lengend Snippet: The comparison of cell surface topography between the unstrained hMSCs, strained hMSCs, and tenocytes, visualized by AFM. (a) Representative AFM height and deflection scans of unstrained hMSCs and 4%, 8%, and 12% strained hMSCs, and tenocytes. In height images, brighter colour indicates higher distance of substrate. In deflection images, the detailed structure of presumably the stress fiber could be observed with AFM in different cell groups. The direction of uniaxial strain was in the red arrow direction. (b) Young's modulus on the cytoskeleton of the cells subjected to 4%, 8%, or 12% cyclic stretching for 72 h as indicated. The ratio was counted by normalizing to the expression amount of corresponding unstrained groups (indicated as 1). Statistical significance ( p < 0.05) was represented by ∗ relative to the unstrained group. n = 3, error bar ± SD.

    Article Snippet: Phase-contrast microscopic images of unstrained and strained hMSCs were obtained (Olympus, Japan) in at least four randomly selected sites from our visual field.

    Techniques: Expressing